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Pagina 7
Immunology 8 : 420 - 431 , 1965 Imura , H . , Y . Nakai , S . Matsukura , Y . Hirata :
The paradoxical binding phenomenon in radioimmunoassay of ACTH . Horm .
Metab . Res . 1974 . To be published . Matsukura , S . , C . D . West , Y . Ichikawa
...
Immunology 8 : 420 - 431 , 1965 Imura , H . , Y . Nakai , S . Matsukura , Y . Hirata :
The paradoxical binding phenomenon in radioimmunoassay of ACTH . Horm .
Metab . Res . 1974 . To be published . Matsukura , S . , C . D . West , Y . Ichikawa
...
Pagina 10
11 - 39 ACTH - 15 - 39 ACTH BF 200 300 400 500 Unlabeled ACTH ( pg ) intact
antisera gamma globulin Fab Per Cent Change in BIF Conventional
Radioimmunoassay ( Antiserum R12x10000 ) o 500 150 100 200 300 400
Unlabeled ...
11 - 39 ACTH - 15 - 39 ACTH BF 200 300 400 500 Unlabeled ACTH ( pg ) intact
antisera gamma globulin Fab Per Cent Change in BIF Conventional
Radioimmunoassay ( Antiserum R12x10000 ) o 500 150 100 200 300 400
Unlabeled ...
Pagina 17
References Abraham G . E . , W . D . Odell , R . Edwards , J . M . Purdy : “ Solid
phase radioimmunoassay of estrogen in ... 342 , 1970 Arends J . : " Comparison
between covalently bound and free antibodies , used for radioimmunoassay " .
References Abraham G . E . , W . D . Odell , R . Edwards , J . M . Purdy : “ Solid
phase radioimmunoassay of estrogen in ... 342 , 1970 Arends J . : " Comparison
between covalently bound and free antibodies , used for radioimmunoassay " .
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ACTH activity added amounts antibody antigen antiserum assay basal Berson binding biological blood bound bovine buffer C-peptide chromatography Clin compared concentration containing cyclic AMP decreased demonstrated determined developed diabetes dilution dose effect Endocr Endocrinology enzymes experiments extract fraction glucagon glucose glucose tolerance growth hormone higher human increase incubation indicated inhibition injection insulin insulin response islets isolated Italy labelled levels LH-RH LHRH liver cells lower material mean measured membranes method minutes normal observed obtained oral pancreas patients peak performed period phase pituitary plasma porcine pork possible preparations present proinsulin protein rabbit radioactivity radioimmunoassay release reported response samples secretion separation serum showed shown significant similar specific stimulation subjects subunit suggest synovial fluid Table tion treatment trypsin tubes University values weight Yalow