Radioimmunoassay: Methodology and Applications in Physiology and in Clinical Studies : Commemorative Issue for Solomon A. BersonRolf Luft, Solomon A. Berson, Rosalyn Sussman Yalow, J. P. Accary Thieme, 1974 - 195 pagine |
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Pagina 40
... separation step ( I ) can be left out when samples con- tain only two of the substances . · Several investigators have used gel filtration for the separation of insulin and proinsulin ( Melani et al . 1970 ; Steiner et al . 1968 ...
... separation step ( I ) can be left out when samples con- tain only two of the substances . · Several investigators have used gel filtration for the separation of insulin and proinsulin ( Melani et al . 1970 ; Steiner et al . 1968 ...
Pagina 62
... separation technique was employed ; under these circumstances , the incubation mixture contained 0.05 M EDTA ; incubations were for 20 hours at 4 ° C , following which anti - rabbit gamma- globulin ( final concentration 1/120 ) with ...
... separation technique was employed ; under these circumstances , the incubation mixture contained 0.05 M EDTA ; incubations were for 20 hours at 4 ° C , following which anti - rabbit gamma- globulin ( final concentration 1/120 ) with ...
Pagina 87
... Separation of free 1251 - GH from 125 I - GH bound to solubilized membrane receptors was at- tempted by use of polyethylene glycol ( Desbuquois and Aurbach 1971 ) , charcoal ( Herbert , Lau , Gottlieb and Bleicher 1965 ) , Sephadex G ...
... Separation of free 1251 - GH from 125 I - GH bound to solubilized membrane receptors was at- tempted by use of polyethylene glycol ( Desbuquois and Aurbach 1971 ) , charcoal ( Herbert , Lau , Gottlieb and Bleicher 1965 ) , Sephadex G ...
Sommario
Methodology | 1 |
Applications to Physiology | 72 |
Tokuko Saito and Kazuo Shizume | 86 |
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Parole e frasi comuni
ACTH activity albumin amylase anti antibody antigen antisera antiserum arginine assay basal binding biological blood glucose bovine buffer C-peptide chymotrypsin chymotrypsinogen clin concentration cross-reactivity cyclic AMP diabetes dilution dose effect eluted Endocr Endocrinology enzymes erythropoietin estradiol estrogen extract fraction Freychet gastrin glucagon glucose tolerance GnRH gonadotropin granulated growth hormone HTSH human immunoassay immunoreactive increase incubation injection insulin levels insulin response islets isolated liver cells labelled LH and FSH LH response LH-RH LHRH luteinizing hormone measured membranes method mg/ml minutes ng/ml normal obesity oral pancreas pancreatic patients peak perifusion pituitary plasma insulin porcine pork preparations progesterone proinsulin proinsulin and insulin protein puberty purified rabbit radioactivity radioimmunoassay receptors releasing hormone S.A. Berson samples Sephadex serum sham feeding specific standard curve steroid stimulation subjects subunit Table theophylline thyroxine tion trypsin unlabeled