Culturing Nerve Cells
A do-it-yourself manual for culturing nerve cells, complete with recipes and protocols.
Because neurons and glia in culture are remarkably similar to those in situ, culture systems make it possible to identify significant cell interactions and to elucidate their mechanisms. This book is in many ways a do-it-yourself manual for culturing nerve cells, complete with recipes and protocols. But it also provides an understanding of the principles behind the protocols. In effect the contributors invite you into their labs and provide much of the information you would obtain from such a visit.The authors of the introductory chapters present the nuts-and-bolts principles of growing nerve cells. The authors of the following chapters discuss the culturing of specific cell types. They explain how their experimental goals have shaped their particular cell culture approach and the advantages and disadvantages of the cell culture systems they have developed. They provide detailed protocols and describe their cultures in practical terms, from when the cells are first plated through the various phases of their development.
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NOrthern blot- BDNF C 4 6 8 10 24 - - - 42 kb - 1.6 kb - 1 kb [+/-] - Ad-CMV-BDNF
D. Ad-CMV-lacz 200 150 - 100 40 so so Time [min] |Ad-CMV-BDNF D. Ad-CMV-
lacz Ad-CMV-NGF H untreated # BDNF-Genotype Figure 4.7 However, there are
Use of an adenovirus-BDNF vector to restore long-term potentiation (LTP) in
slices from BDNF knockout mice. Hippocampal slices were exposed to the Ad-
CMV-BDNF vector immediately after their isolation from BDNF-mutant mice, then
Add 2 ml Fungizone 250 ug/ml, Gibco no. 600-529AE). Divide into 0.5ml aliquots.
Store at -20°C. 3. Neurotrophic factors 7s-NGF (Sigma no. N0513) BDNF (R&D
Systems no. 248-BD, Promega no. G-1491) NT-3 (R&D Systems no. 267-N3 ...
Our results suggest that BDNF may increase the calciumbuffering capacity of
striatal neurons. This finding may have significance in that it has been suggested
that the loss of calciumbuffering capacity associated with loss of calbindin may ...
Figure 14.8 500 pA 5mS V. - BDNF increases functional Na" channel expression
in embryonic striatal neurons. Striatal neurons from E17 rat embryos were
maintained in culture in the absence (–) or presence (+BDNF) of 50ng/ml BDNF
for 7 ...
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