Culturing Nerve Cells
A do-it-yourself manual for culturing nerve cells, complete with recipes and protocols.
Because neurons and glia in culture are remarkably similar to those in situ, culture systems make it possible to identify significant cell interactions and to elucidate their mechanisms. This book is in many ways a do-it-yourself manual for culturing nerve cells, complete with recipes and protocols. But it also provides an understanding of the principles behind the protocols. In effect the contributors invite you into their labs and provide much of the information you would obtain from such a visit.The authors of the introductory chapters present the nuts-and-bolts principles of growing nerve cells. The authors of the following chapters discuss the culturing of specific cell types. They explain how their experimental goals have shaped their particular cell culture approach and the advantages and disadvantages of the cell culture systems they have developed. They provide detailed protocols and describe their cultures in practical terms, from when the cells are first plated through the various phases of their development.
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Dennis Higgins and Gary Banker Regardless of the type of cells to be grown—
neuronal or glial, peripheral or central, ... cell stocks, are described in chapters
devoted to culturing PC12 cells (chapter 6) and to P19 and ES cells (chapter 7).
A number of previous reviews consider the culture and use of PC12 cells (
Greene and Tischler, 1982; Guroff, 1985; Greene et al., 1987; Greene and
Rukenstein, 1989; Halegoua et al., 1991; Shafer, 1991; Teng and Greene, 1994),
and the ...
To reculture the frozen cells, they are quickthawed at 37° in a water bath,
centrifuged, resuspended in complete medium without DMSO, and plated onto
collagen-coated dishes. Because PC12 cells grow best when not at too low a
In one set of studies PC12 cells lacking Trk NGF receptors were transfected with
a series of mutant Trk constructs. Permanent lines expressing these mutant
receptors were generated and analyzed both for signaling capacities and for a
In addition, because the transfected DNA need not integrate into the host
genome to be transcribed, transient transfections are possible both for replicating
and nonreplicating (neuronally differentiated) PC12 cells. Generally, this function
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