Culturing Nerve Cells
A do-it-yourself manual for culturing nerve cells, complete with recipes and protocols.
Because neurons and glia in culture are remarkably similar to those in situ, culture systems make it possible to identify significant cell interactions and to elucidate their mechanisms. This book is in many ways a do-it-yourself manual for culturing nerve cells, complete with recipes and protocols. But it also provides an understanding of the principles behind the protocols. In effect the contributors invite you into their labs and provide much of the information you would obtain from such a visit.The authors of the introductory chapters present the nuts-and-bolts principles of growing nerve cells. The authors of the following chapters discuss the culturing of specific cell types. They explain how their experimental goals have shaped their particular cell culture approach and the advantages and disadvantages of the cell culture systems they have developed. They provide detailed protocols and describe their cultures in practical terms, from when the cells are first plated through the various phases of their development.
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depends on the number of cultures to be prepared; Usually, we use one embryo
per culture dish. Before starting the microdissection, fill one Petri dish (60 x 15
mm, Falcon 3002) with 70% ethanol, six Petri dishes (60 mm) with 10% filtered ...
The volume needed to coat the bottom of a 100-mm Petri dish is ~30ml. Stir the
mixture thoroughly, but try to avoid mixing in air, which forms bubbles. Then pour
the mixture into the Petri dish. Some bubbles probably will be present, but they ...
Sylgard-coated dissection dish. #-inch steel minuten pins (Carolina Biological).
15-ml clear conical centrifuge tubes and rack. 100-mm plastic Petri dishes. 10.
Coarse forceps. 11. Two pairs of watchmaker's forceps (Dumont no. 5). 12.
It is important to avoid plastic fragments in the dishes, because the fragments can
damage the agarose surface. Attaching Coverslips with Sylgard Coverslips with
the agarose coating are attached to the plastic Petri dishes by Sylgard, ...
Box 13.3 Dissection and Culture of Hippocampal Neurons 1. Euthanize the
pregnant rat with halothane or other approved anesthetic, remove uterus, and
place in a sterile Petri dish. The remaining steps are performed in a laminar flow
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