Culturing Nerve Cells
A do-it-yourself manual for culturing nerve cells, complete with recipes and protocols.
Because neurons and glia in culture are remarkably similar to those in situ, culture systems make it possible to identify significant cell interactions and to elucidate their mechanisms. This book is in many ways a do-it-yourself manual for culturing nerve cells, complete with recipes and protocols. But it also provides an understanding of the principles behind the protocols. In effect the contributors invite you into their labs and provide much of the information you would obtain from such a visit.The authors of the introductory chapters present the nuts-and-bolts principles of growing nerve cells. The authors of the following chapters discuss the culturing of specific cell types. They explain how their experimental goals have shaped their particular cell culture approach and the advantages and disadvantages of the cell culture systems they have developed. They provide detailed protocols and describe their cultures in practical terms, from when the cells are first plated through the various phases of their development.
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Using a variety of techniques, we concurrently develop highly purified
populations of Schwann cells and of oligodendrocytes. After reintroduction of
these myelinating cell types into the neuronal cultures, specialized media can be
used to ...
Finally, the solution is diluted with 1 ml of D10, and the cells are plated onto a
PLL-coated Petri dish (PLL, mol. wt. 30,000– 70,000, Sigma no. P-2636, 0.5 mg/
ml, 30 min). The Schwann cell populations obtained are more than 95% pure and
When human fibroblasts contaminating these cultures (even 5% or less) are
exposed to serum in the D10 medium, they divide rapidly and soon overtake the
Schwann cell populations. We have found that a mixture of heregulin (a member
experiments directly demonstrated that the presence of embryo extract in culture
medium allowed ensheathment to occur. Several years later, it was demonstrated
that if the embryo extract was replaced by vitamin C, Schwann cell myelination ...
lamina-Schwann cell interface act together with events at the axon-Schwann cell
interface to induce or regulate gene expression critical for the progress of
myelination. Further work using the neuron-Schwann cell myelinating culture
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