Culturing Nerve Cells
A do-it-yourself manual for culturing nerve cells, complete with recipes and protocols.
Because neurons and glia in culture are remarkably similar to those in situ, culture systems make it possible to identify significant cell interactions and to elucidate their mechanisms. This book is in many ways a do-it-yourself manual for culturing nerve cells, complete with recipes and protocols. But it also provides an understanding of the principles behind the protocols. In effect the contributors invite you into their labs and provide much of the information you would obtain from such a visit.The authors of the introductory chapters present the nuts-and-bolts principles of growing nerve cells. The authors of the following chapters discuss the culturing of specific cell types. They explain how their experimental goals have shaped their particular cell culture approach and the advantages and disadvantages of the cell culture systems they have developed. They provide detailed protocols and describe their cultures in practical terms, from when the cells are first plated through the various phases of their development.
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FLUORESCENT PROBES One of the remarkable advances in the last decade
has been the development of a vast armamentarium of fluorescent probes
suitable for live cell imaging. Among the first probes to be applied widely in
Functional synaptic transmission can be established within the first minute after
contact (Evers et al., 1989). Scale bar; 50 p.m. (C-E) Fluorescence labeling of the
nerve and muscle cells by intracellular injection of rhodamine-dextran into one ...
For definitive identification of neurons with long-distance projections, we have
used in vivo retrograde transport of fluorescent markers to label the neurons prior
to dissociation. To label cells in layer 5 of the cortex that | f - Figure 12.3 Neurons
f - Figure 12.3 Neurons labeled with fluorescent microspheres in vivo (A) and in
vitro (B-E). A. Pyramidal cells in layer 5 of visual cortex are labeled brightly in this
coronal section prepared 2 days after an injection of microspheres in the ...
TRANSPLANTATION OF PURIFIED GRANULE CELLS INTO DEVELOPING
BRAIN To provide a parallel in vivo system, purified granule cells can be
prelabeled with two different fluorescent markers and implanted back into the
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