Culturing Nerve Cells
A do-it-yourself manual for culturing nerve cells, complete with recipes and protocols.
Because neurons and glia in culture are remarkably similar to those in situ, culture systems make it possible to identify significant cell interactions and to elucidate their mechanisms. This book is in many ways a do-it-yourself manual for culturing nerve cells, complete with recipes and protocols. But it also provides an understanding of the principles behind the protocols. In effect the contributors invite you into their labs and provide much of the information you would obtain from such a visit.The authors of the introductory chapters present the nuts-and-bolts principles of growing nerve cells. The authors of the following chapters discuss the culturing of specific cell types. They explain how their experimental goals have shaped their particular cell culture approach and the advantages and disadvantages of the cell culture systems they have developed. They provide detailed protocols and describe their cultures in practical terms, from when the cells are first plated through the various phases of their development.
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Dennis Higgins and Gary Banker Regardless of the type of cells to be grown—
neuronal or glial, peripheral or central, vertebrate or invertebrate—all methods for
preparing dissociated-cell cultures share several features: a source of tissue ...
cells per week. This slow attrition is not of serious concern if the remainder of the
cells are healthy. At some point, when the culture's time has come, cell loss of ...
The morphology of glial cells is much more variable (see Figure 5.1 chapter 18).
High cell density makes it that much more difficult to identify individual cells, as
the length of their processes may be obscured. And glial cells are easy to
overlook when they lie within a dense network. For all these reasons, the
development of ...
Such cultures are the envy of those of us who have chosen to work with other cell
types. ... Culture lore points to the importance of glial cells for the long-term
survival of CNS neurons, and the protocol of Segal et al. follows directly from this
The microisland culture techniques used with sympathetic neurons were difficult
to apply to culturing central neurons because of the properties of central glial
cells, which accompany the neurons in dissociated cell suspensions. The central
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