Culturing Nerve Cells
A do-it-yourself manual for culturing nerve cells, complete with recipes and protocols.
Because neurons and glia in culture are remarkably similar to those in situ, culture systems make it possible to identify significant cell interactions and to elucidate their mechanisms. This book is in many ways a do-it-yourself manual for culturing nerve cells, complete with recipes and protocols. But it also provides an understanding of the principles behind the protocols. In effect the contributors invite you into their labs and provide much of the information you would obtain from such a visit.The authors of the introductory chapters present the nuts-and-bolts principles of growing nerve cells. The authors of the following chapters discuss the culturing of specific cell types. They explain how their experimental goals have shaped their particular cell culture approach and the advantages and disadvantages of the cell culture systems they have developed. They provide detailed protocols and describe their cultures in practical terms, from when the cells are first plated through the various phases of their development.
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Many of these characteristics are believed to reflect the specific endogenous
properties of hippocampal neurons. For example, both the capacity for plasticity
and the susceptibility to anoxia have been linked to the unique properties of
Despite such variations in the stage of development of individual neurons, when
the earliest generated or the most recently generated hippocampal neurons are
labeled selectively with "Hthymidine before being placed into culture, both ...
Other Approaches for Culturing Hippocampal Neurons If the special conditions of
our cultures are unnecessary for your experiments, alternative approaches for
preparing hippocampal cultures are available. Protocols developed in the late ...
Falcon tissueculture plastic dishes provide a consistently good substrate for the
growth of hippocampal cells, when treated with poly-L-lysine according to the
same protocol used for coverslips. They may provide a useful test substrate when
Bahr, B.A., R. L. Neve, J. Sharp, A. I. Geller, and G. Lynch (1994) Rapid and
stable gene expression in hippocampal slice cultures from a defective HSV-1
vector. Mol. Brain Res. 26:277–285. Bailey, C. H., and M. Chen (1988) Long-term
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