Culturing Nerve Cells
A do-it-yourself manual for culturing nerve cells, complete with recipes and protocols.
Because neurons and glia in culture are remarkably similar to those in situ, culture systems make it possible to identify significant cell interactions and to elucidate their mechanisms. This book is in many ways a do-it-yourself manual for culturing nerve cells, complete with recipes and protocols. But it also provides an understanding of the principles behind the protocols. In effect the contributors invite you into their labs and provide much of the information you would obtain from such a visit.The authors of the introductory chapters present the nuts-and-bolts principles of growing nerve cells. The authors of the following chapters discuss the culturing of specific cell types. They explain how their experimental goals have shaped their particular cell culture approach and the advantages and disadvantages of the cell culture systems they have developed. They provide detailed protocols and describe their cultures in practical terms, from when the cells are first plated through the various phases of their development.
Risultati 1-5 di 6
Maintenance of Cultures Typically, cultures are maintained in an incubator that is
perfused automatically with a mixture of air and CO2, the latter at the level
appropriate to the medium being used. The O2 concentration in air is
Embryonic development begins when the eggs are transferred to an egg
incubator. Therefore, it is easy to maintain a continuous supply of embryos at
appropriate stages for culture. Peripheral ganglia are simple to dissect, and each
The embryos begin to develop when the eggs are placed in an egg incubator.
The eggs should be set with their blunt ends up so the embryos will float to the
top and be easy to remove. They do not have to be turned. The temperature in
Let the ganglia sink to the bottom of the tube and transfer them to a centrifuge
tube containing 2 ml warm culture medium (from the Petri dish of culture medium
already in the incubator). Dissociate the ganglia by drawing them into a ...
Transfer tissue pieces into the dish with the papain solution by using a plastic
pipette (with as little CMF-PBS as you can manage) and move into a 35.5°C
incubator for 45 to 60 min. During this time, wash the Lab-Tek well three times
Cosa dicono le persone - Scrivi una recensione