Culturing Nerve Cells
A do-it-yourself manual for culturing nerve cells, complete with recipes and protocols.
Because neurons and glia in culture are remarkably similar to those in situ, culture systems make it possible to identify significant cell interactions and to elucidate their mechanisms. This book is in many ways a do-it-yourself manual for culturing nerve cells, complete with recipes and protocols. But it also provides an understanding of the principles behind the protocols. In effect the contributors invite you into their labs and provide much of the information you would obtain from such a visit.The authors of the introductory chapters present the nuts-and-bolts principles of growing nerve cells. The authors of the following chapters discuss the culturing of specific cell types. They explain how their experimental goals have shaped their particular cell culture approach and the advantages and disadvantages of the cell culture systems they have developed. They provide detailed protocols and describe their cultures in practical terms, from when the cells are first plated through the various phases of their development.
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Cells that have been labeled with a fluorescent dye can be separated from
unlabeled cells by using a fluorescence activated cell sorter (FACS). In this
device, individual cells travel in single file through a laser beam, and their
fluorescence is ...
By the calcium phosphate method (A), few neurons are transfected and brightly
labeled. In panel (A), the labeled axon from the single transfected neuron (
asterisk) surrounds other nearby cells, as shown at higher magnification in the
One successful approach is to plate cells on substrates on which a small, labeled
grid has been imprinted so that they can be identified in the microscope (e.g., see
Stemple et al., 1988). For biochemical purposes in which good optics are not a ...
f - Figure 12.3 Neurons labeled with fluorescent microspheres in vivo (A) and in
vitro (B-E). A. Pyramidal cells in layer 5 of visual cortex are labeled brightly in this
coronal section prepared 2 days after an injection of microspheres in the ...
Thereafter, we label a small aliquot of the cells with a lipophilic dye, such as PKH
-26 or any other marker, plate the labeled cells onto the carpet of unlabeled cells,
and monitor the differentiation of labeled cells. When fluorescent labeling is ...
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