Culturing Nerve Cells
A do-it-yourself manual for culturing nerve cells, complete with recipes and protocols.
Because neurons and glia in culture are remarkably similar to those in situ, culture systems make it possible to identify significant cell interactions and to elucidate their mechanisms. This book is in many ways a do-it-yourself manual for culturing nerve cells, complete with recipes and protocols. But it also provides an understanding of the principles behind the protocols. In effect the contributors invite you into their labs and provide much of the information you would obtain from such a visit.The authors of the introductory chapters present the nuts-and-bolts principles of growing nerve cells. The authors of the following chapters discuss the culturing of specific cell types. They explain how their experimental goals have shaped their particular cell culture approach and the advantages and disadvantages of the cell culture systems they have developed. They provide detailed protocols and describe their cultures in practical terms, from when the cells are first plated through the various phases of their development.
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Despite the limited methods then available, some 25 years before the
development of the phase-contrast microscope, Harrison also visualized the
protoplasmic movement associated with the tips of the elongating fibers,
structures whose ...
Usually, cell attachment is complete within a few hours of plating (perhaps longer
when collagen is used as the substrate), and can be assessed by tapping the
microscope stage (attached cells don't move) or by rinsing away unattached cells
We concentrate here on these issues. MAINTAINING CELLS ON THE
MICROSCOPE STAGE Looking at living cells on a microscope, even for the
simplest of experiments, presents two immediate problems. You must have a
means of getting ...
Marking is achieved by visualizing the colonies at low power on the microscope
and circling their locations on the bottom of the plate with a marker. To isolate
and propagate the colonies, they are located under a dissection microscope
... (2) the cells remain viable for many hours in open air at room temperature on
the microscope stage, ideal for electrophysiological recordings; (3) the
developmental stages of the Xenopus cells in vivo are well-characterized (
Nieuwkoop and ...
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