Culturing Nerve Cells
A do-it-yourself manual for culturing nerve cells, complete with recipes and protocols.
Because neurons and glia in culture are remarkably similar to those in situ, culture systems make it possible to identify significant cell interactions and to elucidate their mechanisms. This book is in many ways a do-it-yourself manual for culturing nerve cells, complete with recipes and protocols. But it also provides an understanding of the principles behind the protocols. In effect the contributors invite you into their labs and provide much of the information you would obtain from such a visit.The authors of the introductory chapters present the nuts-and-bolts principles of growing nerve cells. The authors of the following chapters discuss the culturing of specific cell types. They explain how their experimental goals have shaped their particular cell culture approach and the advantages and disadvantages of the cell culture systems they have developed. They provide detailed protocols and describe their cultures in practical terms, from when the cells are first plated through the various phases of their development.
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By examining the outgrowth of fibers from fragments of frog and chick neural tube
cultured in drops of clotted lymph or plasma, Harrison demonstrated
unequivocally that nerve fibers arise as outgrowths from individual nerve cell
bodies rather ...
In the case of neural lineages, a variety of strategies have been effective for
establishing cell lines. Most aim at creating lines from cycling progenitor cells in
the neural tube or neural crest derivatives. The field of neuronal cell lines is very
Embryos from stage 19 (initial neural tube stage, with neural folds touching each
other, 21 h after fertilization) to stage 22 (suture of neural tube completely closed,
24h after fertilization) are used for nerve-muscle culture. These early stages are ...
For pure neuronal cultures, the dorsal pieces of the embryos are incubated in
Steinberg's solution containing 1 mg/ml collagenase, which results in
dissociation of myotomal muscle cells from the neural tube. The neural tube then
is removed ...
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