Culturing Nerve Cells
A do-it-yourself manual for culturing nerve cells, complete with recipes and protocols.
Because neurons and glia in culture are remarkably similar to those in situ, culture systems make it possible to identify significant cell interactions and to elucidate their mechanisms. This book is in many ways a do-it-yourself manual for culturing nerve cells, complete with recipes and protocols. But it also provides an understanding of the principles behind the protocols. In effect the contributors invite you into their labs and provide much of the information you would obtain from such a visit.The authors of the introductory chapters present the nuts-and-bolts principles of growing nerve cells. The authors of the following chapters discuss the culturing of specific cell types. They explain how their experimental goals have shaped their particular cell culture approach and the advantages and disadvantages of the cell culture systems they have developed. They provide detailed protocols and describe their cultures in practical terms, from when the cells are first plated through the various phases of their development.
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Dennis Higgins and Gary Banker Regardless of the type of cells to be grown—
neuronal or glial, peripheral or central, vertebrate or invertebrate—all methods for
preparing dissociated-cell cultures share several features: a source of tissue ...
First, because almost every method used by neurobiologists has been applied to
characterizing cells in culture, ... With regard to the first part of the chapter, which
concerns characterizing neuronal cultures, specific approaches may differ, but ...
At some point, when the culture's time has come, cell loss of a different sort
begins. Neurons throughout the entire culture begin to degenerate, usually with a
fairly rapid time course, and this process continues until only nonneuronal cells ...
Immunostaining methods for identifying growth cones in neuronal cultures also
have been described (Devoto and Barnstable, 1989; Goslin et al., 1989; figure
5.2). In a similar manner, antibodies against synaptic vesicle proteins can be
the first neurotrophic factor, and the first successful dissociated neuronal cultures
were prepared from chick ganglia. The methods involved in preparing these
cultures are easy, convenient, and inexpensive: Even the embryos come ...
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