Culturing Nerve Cells
A do-it-yourself manual for culturing nerve cells, complete with recipes and protocols.
Because neurons and glia in culture are remarkably similar to those in situ, culture systems make it possible to identify significant cell interactions and to elucidate their mechanisms. This book is in many ways a do-it-yourself manual for culturing nerve cells, complete with recipes and protocols. But it also provides an understanding of the principles behind the protocols. In effect the contributors invite you into their labs and provide much of the information you would obtain from such a visit.The authors of the introductory chapters present the nuts-and-bolts principles of growing nerve cells. The authors of the following chapters discuss the culturing of specific cell types. They explain how their experimental goals have shaped their particular cell culture approach and the advantages and disadvantages of the cell culture systems they have developed. They provide detailed protocols and describe their cultures in practical terms, from when the cells are first plated through the various phases of their development.
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Gary Banker and Kimberly Goslin This second edition of Culturing Nerve Cells,
like the first, is intended for people who want to grow neurons in culture and who
want to do it well! In some senses, it is a do-it-yourself manual complete with ...
Microorganisms reproduce so rapidly that if even only a few “bugs” are
introduced into culture along with your neurons, they will soon win out. This
means that every component involved in preparing cultures must be sterilized,
and its sterility ...
Ann Marie Craig Neurons are difficult to transfect because they are postmitotic
cells; thus, methods that allow entry of DNA into the cytoplasm are not sufficient.
In dividing cells, if one can get the DNA into the cytoplasm, it will enter the
The second half of the chapter, which concerns some of the practical details of
studying neurons in culture, focuses almost exclusively on microscopic methods.
This is not because we believe that this is the only approach for studying cells in
In chapter 11, Mahanthappa and Patterson describe their methods for preparing
cultures of sympathetic neurons from the rat superior cervical ganglion, cultures
that have played a key role in studies of the control of neuronal differentiation.
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