Culturing Nerve Cells
A do-it-yourself manual for culturing nerve cells, complete with recipes and protocols.
Because neurons and glia in culture are remarkably similar to those in situ, culture systems make it possible to identify significant cell interactions and to elucidate their mechanisms. This book is in many ways a do-it-yourself manual for culturing nerve cells, complete with recipes and protocols. But it also provides an understanding of the principles behind the protocols. In effect the contributors invite you into their labs and provide much of the information you would obtain from such a visit.The authors of the introductory chapters present the nuts-and-bolts principles of growing nerve cells. The authors of the following chapters discuss the culturing of specific cell types. They explain how their experimental goals have shaped their particular cell culture approach and the advantages and disadvantages of the cell culture systems they have developed. They provide detailed protocols and describe their cultures in practical terms, from when the cells are first plated through the various phases of their development.
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Oligodendrocytes,. and. 0.2A. Progenitor. Cells. Mark Noble and Margot Mayer-
Pröschel Astrocytes Interest in the biology of the glial cells of the central nervous
system (CNS) has increased enormously in the last two decades, as ever greater
the identification of means of preventing the death of oligodendrocytes. In
analyzing compounds that protect cells against death, we have found it
experimentally useful to distinguish between death caused by exposure to toxic
stimuli (i.e., ...
Using a variety of techniques, we concurrently develop highly purified
populations of Schwann cells and of oligodendrocytes. After reintroduction of
these myelinating cell types into the neuronal cultures, specialized media can be
used to ...
We have developed a technique to prepare oligodendrocytes from mature rat
spinal cord (Rosen et al., 1989) and to observe the formation of myelin sheaths
by the adult oligodendrocytes. This preparation can be undertaken with the use
Abney, E. R., B. P. Williams, and M. C. Raff (1983) Tracing the development of
oligodendrocytes from precursor cells using monoclonal antibodies, fluorescence
-activated cell sorting, and cell culture. Dev. Biol. 100: 166–171. Acheson, A., D.
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