Culturing Nerve Cells
A do-it-yourself manual for culturing nerve cells, complete with recipes and protocols.
Because neurons and glia in culture are remarkably similar to those in situ, culture systems make it possible to identify significant cell interactions and to elucidate their mechanisms. This book is in many ways a do-it-yourself manual for culturing nerve cells, complete with recipes and protocols. But it also provides an understanding of the principles behind the protocols. In effect the contributors invite you into their labs and provide much of the information you would obtain from such a visit.The authors of the introductory chapters present the nuts-and-bolts principles of growing nerve cells. The authors of the following chapters discuss the culturing of specific cell types. They explain how their experimental goals have shaped their particular cell culture approach and the advantages and disadvantages of the cell culture systems they have developed. They provide detailed protocols and describe their cultures in practical terms, from when the cells are first plated through the various phases of their development.
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Exposure of the cells first to fibroblast growth factor, then to nerve growth factor;
causes some to acquire a sympathetic phenotype. More than a dozen cell lines
have been derived in this way from various regions of the CNS, including the ...
to NGF are cessation of proliferation, generation of long neurites, the appearance
of electrical excitability, hypertrophy, and a number of changes in composition
associated with acquisition of a neuronal phenotype. These features have ...
necessary to establish that any observed changes in phenotype arise from the
transfected DNA and are not merely due to the selection of spontaneous variants
in the population. Also, there may be a wide variation among transfected clones
Regulation of neurotransmitter phenotype in sympathetic neurons has been
studied extensively in culture preparations, primarily with neurons from rats (
reviewed by Patterson, 1990; Landis, 1994) but also with neurons from chick
Axons grow under the barriers (hatched areas). See text for details. (Reproduced
with permission from (A) Campenot, 1977, and (B) Pittman, 1985.) prove that
individual sympathetic neurons can change their synaptic phenotype over time in
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