Culturing Nerve Cells
A do-it-yourself manual for culturing nerve cells, complete with recipes and protocols.
Because neurons and glia in culture are remarkably similar to those in situ, culture systems make it possible to identify significant cell interactions and to elucidate their mechanisms. This book is in many ways a do-it-yourself manual for culturing nerve cells, complete with recipes and protocols. But it also provides an understanding of the principles behind the protocols. In effect the contributors invite you into their labs and provide much of the information you would obtain from such a visit.The authors of the introductory chapters present the nuts-and-bolts principles of growing nerve cells. The authors of the following chapters discuss the culturing of specific cell types. They explain how their experimental goals have shaped their particular cell culture approach and the advantages and disadvantages of the cell culture systems they have developed. They provide detailed protocols and describe their cultures in practical terms, from when the cells are first plated through the various phases of their development.
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and fetal calf serum are used widely and readily available from commercial
sources. Fetal calf serum, which is rich in mitogenic factors, usually is the
preferred serum for proliferating cells, cell lines or primary cultures of glia; for
Pretesting of horse serum batches is prudent, as not all are satisfactory for
optimal cell growth and maintenance. At present, consistently good results have
been obtained with “donor” horse serum purchased from JRH Biosciences (
For long-term survival of neurons, this medium must be supplemented with serum
. Typically, we use 5% adult rat serum, prepared in-house, although commercial
lots of calf or horse serum also may be used for this purpose. Three of us (RWB, ...
... contrast to Parkinson's disease, significantly less progress has been. 14. Rat.
Striatal. Neurons. in. Low-Density,. Serum-Free. Culture. Rat Striatal Neurons in
Low-Density, Serum-Free Culture Roseann Ventimiglia and Ronald M Lindsay.
We tested several variations in composition to optimize neuronal survival further,
focusing mainly on the requirements for serum or supplements and the type of
basal medium. At present, we routinely plate the cells in Dulbecco's modified ...
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