Culturing Nerve Cells
A do-it-yourself manual for culturing nerve cells, complete with recipes and protocols.
Because neurons and glia in culture are remarkably similar to those in situ, culture systems make it possible to identify significant cell interactions and to elucidate their mechanisms. This book is in many ways a do-it-yourself manual for culturing nerve cells, complete with recipes and protocols. But it also provides an understanding of the principles behind the protocols. In effect the contributors invite you into their labs and provide much of the information you would obtain from such a visit.The authors of the introductory chapters present the nuts-and-bolts principles of growing nerve cells. The authors of the following chapters discuss the culturing of specific cell types. They explain how their experimental goals have shaped their particular cell culture approach and the advantages and disadvantages of the cell culture systems they have developed. They provide detailed protocols and describe their cultures in practical terms, from when the cells are first plated through the various phases of their development.
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Thus, they no longer represent a tissue, but are in fact cell colonies or cell
cultures. —J. H. Hanks, 1955 Two methods for culturing slices currently are
available: the roller tube technique and the membrane technique. Both methods
can use the ...
During the preparation of slice cultures, utmost care is taken to dissect the tissue
as carefully as possible to avoid damage that would disrupt its histotypic
architecture. The degree of organotypic organization varies with the age of the
This membrane sheath covering can markedly impede the penetration of
substances in the living culture and even of histochemical or
immunocytochemical reagents after culture fixation. Thin organotypic slice
cultures are suited optimally for ...
We have had success with a variety of sizes of tissue slices, including slices of
entire forebrain hemispheres (Baratta et al., ... Living cultures grown on
membranes can be analyzed and processed by many of the same procedures
Convulsant induced discharge in dissociated hippocampal cell cultures appears
significantly different from that in acutely prepared slices or slice cultures,
presumably as a result of their relatively disorganized patterns of connections.
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