Culturing Nerve Cells
A do-it-yourself manual for culturing nerve cells, complete with recipes and protocols.
Because neurons and glia in culture are remarkably similar to those in situ, culture systems make it possible to identify significant cell interactions and to elucidate their mechanisms. This book is in many ways a do-it-yourself manual for culturing nerve cells, complete with recipes and protocols. But it also provides an understanding of the principles behind the protocols. In effect the contributors invite you into their labs and provide much of the information you would obtain from such a visit.The authors of the introductory chapters present the nuts-and-bolts principles of growing nerve cells. The authors of the following chapters discuss the culturing of specific cell types. They explain how their experimental goals have shaped their particular cell culture approach and the advantages and disadvantages of the cell culture systems they have developed. They provide detailed protocols and describe their cultures in practical terms, from when the cells are first plated through the various phases of their development.
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Phenol red interacts with estrogen and prostaglandin receptors (Berthois et al.,
1986; Hubert et al., 1986; Greenberg et al., 1994), and a common impurity in
phenol red affects some ion transporters (Hop and Bunker, 1993). Toxic products
(then toxicity) Yes Yes 11 kb (+)RNA 3 kb ~10° 5-8 h. <1 W68k (not packaged)
cytoplasmic rep. optimal (then toxicity) *Titers can vary easily 10-fold from
estimates here for Crude Cell extracts and almost all can be increased 10- to
100fold by ...
Temperature-sensitive mutants or mutants in accessory genes have been used,
but the best (least toxic) appear to be deletion mutants lacking genes essential
for HSV replication. Deletion mutants and packaging cell lines that express the ...
Crude cell lysates, even those lacking virus, can induce neuron toxicity, in part
via activation of glutamate receptors (Ho et al., 1995). In summary, three
components are needed to make ampliconbased vectors: amplicon plasmid DNA
The titer should be 107-10°pfu/ml. our low-density culture system; it seems that
high-density cultures or tissue can absorb the toxic effects better. The amplicon
vector system is relatively new and certainly is open for improvement. Ideally, one
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