Cells and Tissues in Culture : Methods, Biology and Physiology
Cells and Tissues in Culture: Methods, Biology and Physiology, Volume 1 covers the general fields of tissue culture, including an evaluation of its technique, effects, and contributions to biology. This book focuses on the three methods of culture-tissue culture, cell culture, and organ culture. Other topics include the design of complete synthetic media, possible evolution of the cell types, and energy relationships in growing and stationary cells. The RNA synthesis in cell cultures, culture of amphibian embryonic anlage, action of corticosteroids and adrenaline, and effects of parathyr
eBook, English, 2014
Elsevier Science, Kent, 2014
1 online resource (805 pages)
9781483223001, 1483223000
1058315714
Front Cover; Cells and Tissues in Culture: Methods, Biology and Physiology; Copyright Page; Table of Contents ; Contributors to Volume 1; Foreword to Volume 1; Contents of Volume 2; Contents of Volume 3; Chapter 1. Introduction; References; Chapter 2. Methods; I. Basic Requirements; II. Tissue Culture; III. Cell Culture; IV. Organ Culture; References; Chapter 3. Construction and Use of Synthetic Media; I. Historical Approach; II. Design of Complete Synthetic Media; III. Synthetic Media in Relation to Cells and their Total Environment; IV. Future prospects; References. Chapter 4. Morphological Problems of Cell Type, Shape and IdentificationI. The Problem; II. The Importance of Cell Movement; III. The Main Cell Types; IV. The Significance of the Main Cell Types; V. The Possible Evolution of the Cell Types; VI. Some Physico-chemical Characteristics of the Cell Types; VII. The Problem of Interconversion; VIII. Lessons from the Metaplasia of Naegleria; IX. Cells in Clones and Pure Lines; X. Tissue Culture and the Meaning of Differentiation; References; Chapter 5. The Locomotory Behaviour of Cells; I. Introduction; II. The Individual Cell; III. Cell Populations. I. IntroductionII. Nutritional Requirements of Serially Propagated Cells; III. Amino Acid and Protein Metabolism in Cell Cultures; IV. General Considerations; References; Chapter 9. Amino Acid and Protein Metabolism. II. Further Aspects of the Amino Acid and Protein Metabolism of Tissues in Culture; I. Introduction; II. Nutritional Studies; III. Utilization of Amino Acids; IV. Protein Metabolism; V. Conclusion; References; Chapter 10. Deoxyribonucleic Acid and Ribonucleic Acid Synthesis in Cell Cultures; I. Introduction; II. DNA Synthesis in Cell Cultures; III. RNA Synthesis in Cell Cultures. IV. The Relations between DNA, RNA and Nuclear Protein during the Interphase of Rapidly Dividing CellsReferences; Chapter 11. Mucopolysaccharide Metabolism in Tissue Culture; I. Introduction; II. Analytical and Technical Methods; III. Tissue Culture Studies; IV. Concluding Remarks; References; Chapter 12. Genetic Cytology; I. Introduction; II. Cytogenetics of Man; III. DNA Synthesis and the Cell Cycle; IV. Heterochromatin and Heteropyknosis; V. Sex Chromatin, Sex Chromosomes, Sex Determination; References; Chapter 13. Differentiation: Environmental Factors, Chemical and Cellular
I. Introduction